英文名称：Recombinant Mouse Vascular Endothelial Growth Factor-Sf9
中文简称：【mVEGF Sf9】重组小鼠血管内皮生长因子Sf9

Recombinant Mouse Vascular Endothelial Growth Factor-Sf9 【mVEGF Sf9】重组小鼠血管内皮生长因子Sf9 细胞因子产品描述：
血管内皮生长因子（Vascular endothelial growth factor，VEGF）是血管内皮细胞特异性的肝素结合生长因子（heparin-binding growth factor），可在体内诱导血管新生。VEGF有六种不同亚型，包括VEGF-A，-B，-C，-D，及-E；其分子量从35至44kDa不等，每个亚型特异性地与三个“血管内皮生长因子受体”（VEGFR-1，-2，及-3)的特定组合相结合。VEGF是高度保守的同源二聚体糖蛋白。二条分子量各为24kDa的单链以二硫键组成二聚体。VEGF分解的单体无活性,去除N 2糖基对生物效应无影响，但可能在细胞分泌中起作用。由于mRNA不同的剪切方式，产生出分别VEGF121、VEGF145、VEGF165、VEGF185、VEGF206等至少5种蛋白形式，其中VEGF121、VEGF145、VEGF165是分泌型可溶性蛋白，能直接作用于血管内皮细胞促进血管内皮细胞增殖，增加血管通透性。
小鼠血管内皮生长因子Sf9重组蛋白是在杆状病毒-昆虫Sf9细胞中重组表达的，是由两条糖基化的多肽链构成。每条链包含有164个氨基酸，分子量为48kDa。该小鼠血管内皮生长因子Sf9重组蛋白最终由标准的色谱技术纯化出。
来源宿主---杆状病毒-昆虫Sf9细胞中重组表达

Recombinant Mouse Vascular Endothelial Growth Factor-Sf9 【mVEGF Sf9】重组小鼠血管内皮生长因子Sf9 细胞因子产品使用须知：
溶解：提供的小鼠血管内皮生长因子Sf9(mVEGF Sf9)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml小鼠血管内皮生长因子Sf9(mVEGF Sf9)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释。
保存：小鼠血管内皮生长因子Sf9(mVEGF Sf9)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Vascular Endothelial Growth Factor-Sf9 【mVEGF Sf9】重组小鼠血管内皮生长因子Sf9 细胞因子产品英文描述：

Synonyms 

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction 

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description 

Vascular Endothelial Growth Factor Mouse Recombinant produced in Sf9 insect cells is a double, glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 48 kDa.
The VEGF is purified by proprietary chromatographic techniques.

Source  （来源）

Baculovirus Sf9 cells.

Physical Appearance  （表面及外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

Solubility  （溶解性）

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor-Sf9 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability    （稳定性）

Lyophilized Vascular Endothelial Growth Factor Sf9 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-Sf9 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity      （纯度）

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity   （生物活性）

The ED50 range, determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (measured by 3H-thymidine uptake) is 1-2 ng/ml, corresponding to a specific activity of 1x106 Units/mg.

Usage 

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.