Recombinant Mouse Macrophage Colony Stimulating Factor
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英文名称:Recombinant Mouse Macrophage Colony Stimulating Factor
中文简称:【mM CSF】重组小鼠巨噬细胞集落刺激因子
产品链接:重组小鼠巨噬细胞集落刺激因子
Recombinant Mouse Macrophage Colony Stimulating Factor 【mM CSF】重组小鼠巨噬细胞集落刺激因子 细胞因子产品描述:
巨噬细胞集落刺激因子(Macrophage colony-stimulating factor,M-CSF),也称集落刺激因子-1(CSF-1),是近年来受到广泛重视的具有多种生物学功能的细胞因子。M-CSF对单核细胞的增殖、分化及维持其活性有重要作用。另外它还调节胎盘功能,促进骨吸收。M-CSF为由链间二硫键连接而成的同源二聚体糖蛋白。广泛存在于血清、尿及其他组织中。人M-CSF与小鼠M-CSF在其DNA序列上有70%的同源性。M-CSF可以在间质细胞如成纤维细胞、成骨细胞及内皮细胞中合成,也可在激活的巨噬细胞、B细胞、T细胞及多种肿瘤细胞产生。
小鼠巨噬细胞集落刺激因子重组蛋白是在大肠杆菌中重组表达的,是一条由二硫键连接两条非糖基化多肽链形成的同源二聚体,包含有2 x 156个氨基酸,总分子量为36.4kDa。该小鼠巨噬细胞集落刺激因子重组蛋白最终由标准的色谱技术纯化出。
来源宿主---大肠杆菌中重组表达
Recombinant Mouse Macrophage Colony Stimulating Factor 【mM CSF】重组小鼠巨噬细胞集落刺激因子 细胞因子产品使用须知:
溶解:提供的小鼠巨噬细胞集落刺激因子(mM CSF)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml小鼠巨噬细胞集落刺激因子(mM CSF)的5mM Tris,pH 8.0 ,100mM NaCl溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:小鼠巨噬细胞集落刺激因子(mM CSF)在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装后置于-18℃冻存。请避免反复冻融。
Recombinant Mouse Macrophage Colony Stimulating Factor 【mM CSF】重组小鼠巨噬细胞集落刺激因子 细胞因子产品英文描述:
Synonyms
CSF-1, Lanimostim, MCSF, M-CSF.
Introduction
Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Description
Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.
Source (来源)
Escherichia Coli.
Physical Appearance (物理特征)
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized with 0.5xPBS, pH 8.0.
Solubility (稳定性)
It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Amino acid sequence (氨基酸序列)
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Purity (纯度)
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity (生物活性)
The ED50, as calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells is typically 1.1-1.6ng/ml corresponding to 909,090-625,000 U/mg.
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