人源肝脏细胞及iPS向肝脏细胞定向分化的解决方案提供者

iPS向肝脏细胞定向分化操作系统

(Cellartis iPS Cell to Hepatocyte Differentiation System)

以肝脏细胞为模型的实验越来越受到重视，在肝脏病变机理研究、药物代谢研究、药物毒理评估等方面有广阔的应用前景。然而，以个体原代肝细胞为材料来源会产生如取材困难、材料批间差大、无法长期稳定供应等一系列难以克服的问题。因此，通过iPS批量转化生产肝细胞的方法就显示出特别的优势。Cellartis iPS Cell to Hepatocyte Differentiation System可以稳定地、规模化地实现iPS向hepatocyte的诱导转化生产，而且诱导转化出的肝细胞能够稳定表达药物代谢相关酶系统和药物转运系统。

Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系统，客户既可以对某个特殊病人来源的iPS实现转化，也可以利用cellartis供应的iPS进行标准化操作。使用本产品的过程起始于诱导人iPS转化成为确定性内胚层（Definitive Endoderm (DE)）细胞，然后诱导转化DE至肝细胞。本产品是All-In-One型产品，实验所需的培养基、基质（coating）等包括在内。应用本产品转化约3 × 10⁶ hiPS cells,可以获得约5 × 10⁶ 肝细胞（大约50 cm²面积的单层贴壁细胞）。

利用Cellartis iPS Cell to Hepatocyte Differentiation System转化得到的肝细胞除了清晰表现出肝细胞的必备特征外（如肝细胞特异性标志物、CYP系列酶等），还保留了原始细胞供体者的遗传基因背景。另外，相比较从组织切除获得的原代肝脏细胞（通常需要低温保存），通过本产品系统转化获得的肝细胞在更长的时间跨度上保持着生理功能的稳定性。这一点对于毒理学评估和病毒感染评估具有显著的意义。为长时间维持培养hiPS转化而来的肝细胞，我们推荐Cellartis Hepatocyte Maintenance Medium (Cat. No. Y30051)。

关于Cellartis 卓越的iPS Cell to Hepatocyte Differentiation技术的专项说明，欢迎来电咨询。

■ Cellartis iPS Cell to Hepatocyte Differentiation System 产品特点

· Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS

   cell lines, so no need to optimize for your lines.

· Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS

   cell-derived hepatocytes with diverse genetic backgrounds.

· Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver

   disease models.

· Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

· Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

■ 产品成分

· Cellartis DEF-CS 100 Culture System (Y30020, not sold separately)

· Cellartis Definitive Endoderm Differentiation Kit (Y30030, not sold separately)

· Cellartis Hepatocyte Differentiation Kit (Y30050)

Code No. Product    Size

Y30055     Cellartis® iPS Cell to Hepatocyte Differentiation System        1 Kit

Y30051     Cellartis® Hepatocyte Maintenance Medium          100 ml

产品实验图：

Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

Cellartis iPS Cell to Hepatocyte Differentiation System 产品订购清单

Y30050     Cellartis® Hepatocyte Differentiation Kit                          

Y30051     Cellartis® Hepatocyte Maintenance Medium                           

Y30035     Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™                            

组份-1      Cellartis DEF-CS 100 Culture System (Cat. No. Y30020                    

组份-2      Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030                          

Y30055     Cellartis DEF-CS 100 Culture System (Cat. No. Y30020,                  

         Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030                          

         Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050                       

“Cat. No. Y30020 Package Contents

Package 1:

· 100 ml DEF-CS 100 Basal Medium

· 800 μl DEF-CS COAT-1

Package 2:

· 300 μl DEF-CS GF-1

· 100 μl DEF-CS GF-2

· 40 μl DEF-CS GF-3

Cat. No. Y30030 Package Contents

 10 ml Definitive Endoderm Differentiation Coating

 18 ml Definitive Endoderm Differentiation Day 0

 18 ml Definitive Endoderm Differentiation Day 1

 18 ml Definitive Endoderm Differentiation Day 2

 25 ml Definitive Endoderm Differentiation Day 3

 47 ml Definitive Endoderm Differentiation Day 4

 47 ml Definitive Endoderm Differentiation Day 6

Cat. No. Y30050 Package Contents

 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)

 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)

 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)

 1 bottle Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)

 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)

 1 tube Hepatocyte Coating (7.5 ml)”                            

参考文献：

Asplund, Annika, et al.One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev and Re