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产品编号:HY-001463

BioGems鼠β-NGF预包被ELISA试剂盒,Murine β-NGF Pre-Coated ELISA Kit

Murine β-NGF Pre-Coated ELISA Kit

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  • 生产厂家: BioGems
  • 订购货号:
  • 规格型号: 1 Kit
  • 产品编号: HY-001463
  • 产品种类: 细胞活力检测

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产品描述 说明书【下载】 用户评论【0】

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.

The BioGems Murine β-NGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure β-NGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for β-NGF. It contains NS0-expressed recombinant β-NGF. The detection antibody is a biotinylated goat polyclonal antibody specific for β-NGF. The kit is analytically validated with ready to use reagents.

 

β-NGF是与BDNFNT-3NT-4蛋白结构相关的神经营养因子。这些蛋白属于可能与稳定二聚体结构相关的半胱氨酸结家族生长因子。 β-NGF是通过其受体β-NGFR传导信号的强效神经营养因子,并且在感觉和交感神经系统的发育和保存中起关键作用。 β-NGF还充当B淋巴细胞的生长和分化因子,并增强B细胞存活。

BioGemsβ-NGF预包被ELISA(酶联免疫吸附试验)试剂盒采用固相免疫测定试剂盒,专门设计用于检测β-NGF,其中96微孔板预包被β-NGF特异小鼠单克隆抗体NGF。它含有NS0表达的重组β-NGF。检测抗体是对β-NGF特异的生物素化山羊多克隆抗体。该试剂盒经过分析验证,是即用型试剂。

 

Product Details

 

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<1pg/ml

Range:

31.2pg/ml-2000pg/ml

Specificity:

Natural and recombinant Murine β-NGF

Uniprot:

P01139

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 – 8 .

 

Components组份

 

Description

Quantity

Volume

Anti-Murine β-NGF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine β-NGF Standard

2

10 ng vial

Murine β-NGF Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

 

Reproducibility 可重复性

 

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

136

727

1326

153

839

1645

Standard Deviation

5.3

41.44

83.54

6.58

53.7

121.73

Consistency (%CV)

3.9

5.7

6.3

4.3

6.4

7.4

窗体底端

 

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