英文名称：Recombinant Human Platelet Derived Growth Factor-BB，Yeast
中文简称：【PDGF BB Yeast】重组人血小板衍生生长因子-BB,酵母表达

Recombinant Human Platelet Derived Growth Factor-BB，Yeast 【PDGF BB Yeast】重组人血小板衍生生长因子-BB,酵母表达 细胞因子产品描述：

血小板衍生生长因子（Platelet Derived Growth Factor，PDGF）是由两条相关多肽链通过二硫键连接而成的二聚体。PDGF 贮存于血小板α颗粒中的一种碱性蛋白质，是一些细胞，如纤维母细胞、平滑肌细胞、神经胶质细胞的促细胞分裂剂。PDGF可分为PDGF-I和PDGF-II，二者所含蛋白质相同，仅糖苷含量各异，它们是由两个不同的多肽链由二硫键结合而成的二聚体，其中A链较大(PDGF-I中的A链为17 000，PDGF-II中的A链为15 000)，B链较小(14 000)。由血小板纯化得到的PDGF为AB、BB双聚体，许多肿瘤细胞中的PDGF为AA双聚体。目前认为PDGF在伤口愈合、胆固醇与磷酯合成的调节、动脉粥样硬化等多种生理及病理过程中起重要作用。
人血小板衍生生长因子-BB，酵母表达重组蛋白是在酿酒酵母中重组表达的，是由两条糖基化多肽链组成的同源二聚体，包含有109个氨基酸，分子量为32，021Da。该人血小板衍生生长因子-BB，酵母表达重组蛋白最终由标准的色谱技术纯化出。
来源宿主---酿酒酵母中重组表达

Recombinant Human Platelet Derived Growth Factor-BB，Yeast 【PDGF BB Yeast】重组人血小板衍生生长因子-BB,酵母表达 细胞因子产品使用须知：
溶解：提供的人血小板衍生生长因子-BB,酵母表达(PDGF BB Yeast)产品为经0.2um孔径过滤灭菌的白色冻干粉末；该冻干粉末是从含人血小板衍生生长因子-BB,酵母表达(PDGF BB Yeast)的20mM 乙酸钠（不含任何载体蛋白）溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释。
保存：人血小板衍生生长因子-BB,酵母表达(PDGF BB Yeast)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装后置于-18℃冻存。请避免反复冻融。

Recombinant Human Platelet Derived Growth Factor-BB，Yeast 【PDGF BB Yeast】重组人血小板衍生生长因子-BB,酵母表达 细胞因子产品英文描述：
Synonyms 

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.

Introduction  （介绍）

PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.

Description  

Platelet-Derived Growth Factor BB Human Recombinant is a glycosylated homodimer produced in Saccharomyces cerevisiae, containing 2x109 amino acids and having a molecular mass of 32kDa.
PDGF-BB is purified by proprietary chromatographic techniques.

Source 

Saccharomyces cerevisiae.

Physical Appearance  (外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The PDGF-BB was lyophilized from 0.2 µm filtered solution in 20mM sodium phosphate in absence of any carrier protein.

Solubility  （溶解性）

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability   （稳定性）

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity   （纯度）

Greater than 98.0% as determined by SDS-PAGE.

Amino acid sequence   （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu.

Biological Activity   （生物活性）

The ED50, as measured by its ability to stimulate proliferation of mouse 3T3 fibroblasts is typically 1 ng/ml, corresponding to a Specific Activity of 1,000,000IU/mg.

Usage 

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.