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ProSpec Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重组人白介素-1α

Recombinant Human Interleukin-1 alpha

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  • 生产厂家: ProSpec
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  • 保存温度: -18℃
  • 规格型号: 2ug,10ug,1mg
  • 产品编号: HY-001124
  • 产品种类: 特定细胞专用培养基

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英文名称:Recombinant Human Interleukin-1 alpha
中文简称:【IL 1 alpha】重组人白介素-1α

 

Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重组人白介素-1α 细胞因子产品描述:

白细胞介素-1(interleukin-1,IL-1)是在感染和炎症状态下,由多种细胞产生的、有多方面生物学功能的细胞因子,其生物学作用十分广泛,主要包括参与介导炎症反应、调节免疫、调节机体代谢。IL-1分子重量为17.5KD,有两种类型:IL-1α和IL-1β,主要以IL-1β为主。IL-1受体(interleukin-1 receptor,IL-1R)包括Ⅰ型和Ⅱ型,此外还存在一种内源性IL-1受体拮抗剂 (interleukin-1 receptor antagonist,IL-1ra或IL-1γ),IL-1ra可以与IL-1受体结合,但是不引起相应的作用。
人白介素-1α重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有159个氨基酸,分子量为18022Da。该人白介素-1α重组蛋白最终由标准的色谱技术纯化出。
来源宿主---大肠杆菌中重组表达

 

Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重组人白介素-1α 细胞因子产品使用须知:

溶解:提供的人白介素-1α(IL 1 alpha)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml人白介素-1α(IL 1 alpha)的20mM Tris-HCL(pH 8),5mM MgCl2 和10%甘油溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人白介素-1α(IL 1 alpha)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装后置于-18℃冻存。请避免反复冻融。

 

Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重组人白介素-1α 细胞因子产品英文描述:

Synonyms 

Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.


Introduction 

IL-1 alpha is produced by activated macrophages, stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1A proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.


Description 

Interleukin-1 alpha Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton.
The IL-1A is purified by proprietary chromatographic techniques.


Source 

Escherichia Coli.


Physical Appearance   (外观)

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation 

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 25mM Tris-HCl, pH 8.


Solubility   (溶解性)

It is recommended to reconstitute the lyophilized Interleukin 1 alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Stability     (稳定性)

Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1a should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.


Purity    (纯度)

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Amino acid sequence    (氨基酸序列)

SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAV KFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITG SETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILE NQA.


Biological Activity     (生物活性)

The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a Specific Activity of 1 x 109 IU/mg.


Protein content     (蛋白质含量)

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.


Usage 

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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