Human MIP-3β (CCL19) Pre-Coated ELISA Kit
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MIP-3β是一种CC趋化因子,在胸腺,淋巴结和活化的骨髓基质细胞中表达,通过CCR7受体传导。 MIP-3β是T和B淋巴细胞和骨髓祖细胞的趋化因子。
BioGems人MIP-3β(CCL19)预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫法,专门设计用于检测MIP-3β(CCL19),96孔微板预包被MIP-3β特异鼠单克隆抗体(CCL19)。它含有大肠杆菌表达的重组MIP-3β(CCL19)。检测抗体是MIP-3β特异生物素化山羊多克隆抗体(CCL19)。该试剂盒是即用型试剂盒,已经过分析验证。
Human MIP-3β (CCL19) Pre-Coated ELISA Kit分析
Product Details
Assay Type: |
Solid Phase Sandwich ELISA: 96-Well Strip Plate |
Sensitivity: |
<10pg/ml |
Range: |
15.6pg/ml-1000pg/ml |
Specificity: |
Natural and recombinant Human MIP-3β (CCL19) |
Uniprot: |
Q6IBD6 |
Interference: |
No detectable cross-reactivity with other relevant proteins |
Storage Conditions: |
Store the unopened product at 2 – 8 ℃. |
Components组份
Description |
Quantity |
Volume |
Anti-Human MIP-3β(CCL19) Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human MIP-3β (CCL19) Standard |
2 |
10 ng vial |
Human MIP-3β (CCL19) Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility 可重复性
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
97 |
293 |
583 |
117 |
374 |
627 |
Standard Deviation |
5.53 |
14.1 |
36.15 |
7.5 |
18.7 |
42.64 |
Consistency (%CV) |
5.7 |
4.8 |
6.2 |
6.4 |
5 |
6.8 |
Catalog Number : BGK6IBD6
Description
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Human MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.
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