Human RANTES Pre-Coated ELISA kit
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RANTES是CC趋化因子,可通过CCR1,CCR3,CCR5和US28(巨细胞病毒受体)受体传导信号。 它是一种趋化单核细胞,记忆T细胞(CD4 + / CD45RO),嗜碱性粒细胞和嗜酸性粒细胞的趋化剂。RANTES还具有抑制某些HIV-1,HIV-2和猿免疫缺陷病毒(SIV)的能力。
BioGems人类RANTES预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫测定法,专门设计用于检测RANTES,96孔板预包被RANTES特异性小鼠单克隆抗体。它含有大肠杆菌表达的重组RANTES。检测抗体是RANTES特异性的生物素化山羊多克隆抗体。该试剂盒已经过分析验证,是即用型试剂。
Product Details
Assay Type: |
Solid Phase Sandwich ELISA: 96-Well Strip Plate |
Sensitivity: |
<3pg/ml |
Range: |
15.6pg/ml-1000pg/ml |
Specificity: |
Natural and recombinant Human Rantes |
Uniprot: |
D0EI67 |
Interference: |
No detectable cross-reactivity with other relevant proteins |
Storage Conditions: |
Store the unopened product at 2 – 8 ℃. |
Components
Description |
Quantity |
Volume |
Anti-Human Rantes Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human Rantes Standard |
2 |
10 ng vial |
Human Rantes Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
147 |
293 |
501 |
110 |
339 |
658 |
Standard Deviation |
5.88 |
15.53 |
31.1 |
8.14 |
24.41 |
56.6 |
Consistency (%CV) |
4 |
5.3 |
6.2 |
7.4 |
7.2 |
8.6 |
Description
RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).
The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.
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