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BioGems 人RANTES 预包被ELISA试剂盒,Human RANTES Pre-Coated ELISA kit

Human RANTES Pre-Coated ELISA kit

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  • 生产厂家: BioGems
  • 订购货号:
  • 规格型号: 1 Kit
  • 产品编号: HY-001459
  • 产品种类: 细胞活力检测

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客服电话 150 2101 0459

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RANTESCC趋化因子,可通过CCR1CCR3CCR5US28(巨细胞病毒受体)受体传导信号 它是一种趋化单核细胞,记忆T细胞(CD4 + / CD45RO),嗜碱性粒细胞和嗜酸性粒细胞的趋化剂。RANTES还具有抑制某些HIV-1HIV-2和猿免疫缺陷病毒(SIV能力。

BioGems人类RANTES预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫测定法,专门设计用于检测RANTES96孔板预包被RANTES特异性小鼠单克隆抗体。它含有大肠杆菌表达的重组RANTES。检测抗体是RANTES特异性的生物素化山羊多克隆抗体。该试剂盒经过分析验证,是即用型试剂。

 

 

Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<3pg/ml

Range:

15.6pg/ml-1000pg/ml

Specificity:

Natural and recombinant Human Rantes

Uniprot:

D0EI67

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 – 8 .

 

Components

Description

Quantity

Volume

Anti-Human Rantes Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human Rantes Standard

2

10 ng vial

Human Rantes Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

 

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

147

293

501

110

339

658

Standard Deviation

5.88

15.53

31.1

8.14

24.41

56.6

Consistency (%CV)

4

5.3

6.2

7.4

7.2

8.6

 

Description

RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).

The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.

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