Basement Membrane Matrix, 10ml *LDEV-Free
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产品英文名称:Basement Membrane Matrix, 10ml *LDEV-Free
产品中文名称:基底膜基质胶,10ml,不含LDEV
BD Matrigel™ Basement Membrane Matrix产品介绍:
BD Matrigel 是从富含胞外基质蛋白的EHS小鼠肿瘤中提取出的基底膜基质胶,主要成份有IV型胶原,层粘连蛋白,硫酸肝素糖蛋白,巢蛋白。另外,还包含TGF-beta,表皮生长因子,胰岛素样生长因子,成纤维细胞生长因子,组织纤溶酶原激活物和其它生长因子。
BD Matrigel™基底膜基质胶可以用于有效培养常规细胞、转变的锚定依赖上皮细胞和其它类型细胞。这些细胞包括神经细胞,支持细胞,click lens,血管内皮细胞和肝细胞。BD Matrigel还影响成年小鼠肝细胞基因表达,同时也影响三维培养基中小鼠和人乳腺上皮细胞。BD Matrigel基底膜基质胶支持体内末梢神经再生,可用于新陈代谢和病理学研究,是几种特有类型肿瘤细胞发病检测的基础。BD Matrigel作为培养基还可用于体内和体外血管再生研究。 另外,BD Matrigel有利于人肿瘤细胞在无免疫性老鼠体内扩散。
BD Matrigel™ 产品应用:
» 细胞生长和分化
» 新陈代谢/病毒学研究
» 肿瘤细胞侵袭检测
» 体内和体外血管再生检测
» 体内血管再生研究和无免疫鼠中肿瘤扩散
Basement Membrane Matrix, 5 ml *LDEV-Free英文简述:
BD Matrigel™ Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelioid and other cell types. These include neurons, Sertoli cells, chick lens, and vascular endothelial cells, and hepatocytes. BD Matrigel will influence gene expression in adult rat hepatocytes as well as three dimensional culture in mouse and human mammary epithelial cells. It will support in vivoperipheral nerve regeneration, can be used for metabolism and toxicology studies, and is the basis for several types of tumor cell invasion assays. BD Matrigel provides the substrate necessary for the study of angiogenesis both in vitro and in vivo. BD Matrigel also supports in vivopropagation of human tumors in immunosupressed mice.
Quality
• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations are determined by Lowry method
• Endotoxin units are measured by Limulus Amoebocyte Lysate assay
• BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
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