EasySep™ Human Buffy Coat CD14 Positive Selection Kit
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产品英文名称: EasySep™ Human Buffy Coat CD14 Positive Selection Kit
产品中文名称: EasySep™ 人白膜CD14阳性选择试剂盒
EasySep™ Human Buffy Coat CD14 Positive Selection Kit 产品英文描述
The EasySep™ Human Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.
EasySep™ 人白膜CD14阳性选择试剂盒 Recommended for
The isolation of human CD14+ cells from buffy coat by positive selection
EasySep™ 人白膜CD14阳性选择试剂盒产品组成
• EasySep™ Human Buffy Coat CD14 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
• EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
EasySep™ 人白膜CD14阳性选择试剂盒保存方法
EasySep® Human Buffy Coat CD14 Positive Selection Cocktail.
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep® Whole Blood Magnetic Nanoparticles.
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep® RBC Lysis Buffer 10X Concentrate
10X Concentrate is stable at room temperature for 2 years. Store at room temperature. 1X Lysis Buffer is stable at 4°C for 3 months. Store at 2 - 8°C. Do not freeze.
EasySep™ 人白膜CD14阳性选择试剂盒实验资料
FACS Histogram Results Using EasySep® Human Buffy Coat CD14 Positive Selection Kit
Starting with buffy coat, the CD14+ cell content of the selected fraction typically ranges from 96.5% - 99.6%.*Red blood cells were removed by lysis prior to flow cytometry.
Typical EasySep™ Procedure for Human Cells
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For research use only. Not intended for human or animal diagnostic or therapeutic uses.
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